Visualization of Nuclei in of the Number of NucleiAspergilins oJ : yzae in Each Conidiumwith EGFP and Analysis by FACS JSBA

fiuorescent spot in the A. nidulans transformant, approximately 66% of conidia had two, 24% had one, and 10% had three or more in the A. oi:yzae transformant. The conidia expressing H2B::EGFP werc put through FACS (fluorescence-activated cell sorting> analysis and two sharp peaks, corresponding to one and two nuclei in each co"idium, were noted in the A. ot:yzae transformant. In addition, the A. ot:yzae uninucleate cenidia that were successfully iselated by FACS reproduced conidia with a]most the same number distribution of nuclei as that of the original. Conidia of fiye A. oi:yzae strains used in sake brewing were scored for the number of nuclei, showi"g that a yaried number of nuclei existed in each conidium and some strains had a small number of uninucleate conidia.